Update README.md

README.md

@@ -9,7 +9,7 @@ This script scans an input fasta file for sequences that match or are complement
 python (v3.3.2)
 
 ## Overview
-In _ G-rich motifs within phosphorothioate-based antisense oligonucleotides (ASOs) drive activation of FXN expression through indirect effects_ we showed that antisense oligonucleotides (ASOs) that were carefully designed can still have off-target effects. This script scans an input fasta file and calculates a pairing score based on the provided position-specific score matrix (pssm) that can be customized according to the researchers needs.
+In _ G-rich motifs within phosphorothioate-based antisense oligonucleotides (ASOs) drive activation of FXN expression through indirect effects_ we showed that antisense oligonucleotides (ASOs) that were carefully designed can still have off-target effects. This script scans an input fasta file containing multiple features and calculates a pairing score based on the provided position-specific score matrix (pssm) that can be customized according to the researchers needs.
 
 An example of a pssm is provided [here](https://github.com/thepailab/aso_pairing_score/blob/02c6e8ae2c33e1fdacdd88d0f6d32e42662c08f4/S30.PSSM). Each column represents a base on the ASO and each row the matching nucleotide, thus, each cell is the assigned score for finding that nucleotide at a given ASO position.
 
@@ -32,12 +32,12 @@ optional arguments:
 
 | Column Name  | Description |
 | ------------- | ------------- |
-| chromosome  | Content Cell  |
-| pssm_start  | Content Cell  |
-| pssm_end  | Content Cell  |
-| seq  | Content Cell  |
-| pssm_score  | Content Cell  |
-| strand  | Content Cell  |
+| feature  | name of the feature used to blast the ASO  |
+| pssm_start  | start of the feature targeted by the ASO |
+| pssm_end  | end of the feature targeted by the ASO  |
+| seq  | targeted sequence  |
+| pssm_score  | summed pairing score between the ASO and the feature  |
+| strand  | strand used to calculate the score  |