idealImmunoTP - Interactive Differential Expression AnaLysis in RNA-seq data

idealImmunoTP is a Bioconductor package containing a Shiny application for analyzing RNA-Seq data in the context of differential expression. This enables an interactive and at the same time reproducible analysis, keeping the functionality accessible, and yet providing a comprehensive selection of graphs and tables to mine the dataset at hand.

idealImmunoTP is an R package which fully leverages the infrastructure of the Bioconductor project in order to deliver an interactive yet reproducible analysis for the detection of differentially expressed genes in RNA-Seq datasets. Graphs, tables, and interactive HTML reports can be readily exported and shared across collaborators. The dynamic user interface displays a broad level of content and information, subdivided by thematic tasks. All in all, it aims to enforce a proper analysis, by reaching out both life scientists and experienced bioinformaticians, and also fosters the communication between the two sides, offering robust statistical methods and high standard of accessible documentation.

It is structured in a similar way to the pcaExplorer, also designed as an interactive companion tool for RNA-seq analysis focused rather on the exploratory data analysis e.g. using principal components analysis as a main tool.

The interactive/reactive design of the app, with a dynamically generated user interface makes it easy and immediate to apply the gold standard methods in a way that is information-rich and accessible also to the bench biologist, while also providing additional insight also for the experienced data analyst. Reproducibility is supported via state saving and automated report generation.

Installation

ideal can be easily installed using BiocManager::install():

if (!requireNamespace("BiocManager", quietly=TRUE))
    install.packages("BiocManager")
    
BiocManager::install("GenomeInfoDbData")
install.packages("devtools")

devtools::install_github("baj12/idealImmunoTP", dependencies = T)

library(idealImmunoTP)

idealImmunoTP()

or, optionally,

BiocManager::install("federicomarini/ideal")
# or alternatively...
devtools::install_github("federicomarini/ideal")

Quick start

This command loads the idealImmunoTP package

library("idealImmunoTP")

The main parameters for idealImmunoTP are

• dds_obj - a DESeqDataSet object. If not provided, then a countmatrix and a expdesign need to be provided. If none of the above is provided, it is possible to upload the data during the execution of the Shiny App
• res_obj - a DESeqResults object. If not provided, it can be computed during the execution of the application
• annotation_obj - a data.frame object, with row.names as gene identifiers (e.g. ENSEMBL ids) and a column, gene_name, containing e.g. HGNC-based gene symbols. If not provided, it can be constructed during the execution via the org.eg.XX.db packages
• countmatrix - a count matrix, with genes as rows and samples as columns. If not provided, it is possible to upload the data during the execution of the Shiny App
• expdesign -a data.frame containing the info on the experimental covariates of each sample. If not provided, it is possible to upload the data during the execution of the Shiny App

The idealImmunoTP app can be launched in different modes:

• idealImmunoTP(dds_obj = dds, res_obj = res, annotation_obj = anno), where the objects are precomputed in the current session and provided as parameters
• idealImmunoTP(dds_obj = dds), as in the command above, but where the result object is assembled at runtime
• idealImmunoTP(countmatrix = countmatrix, expdesign = expdesign), where instead of passing the defined DESeqDataSet object, its components are given, namely the count matrix (e.g. generated after a run of featureCounts or HTSeq-count) and a data frame with the experimental covariates. The design formula can be constructed interactively at runtime
• idealImmunoTP(), where the count matrix and experimental design can simply be uploaded at runtime, where all the derived objects can be extracted and computed live. These files have to be formatted as tabular text files, and a function in the package tries to guess the separator, based on heuristics of occurrencies per line of commonly used characters

Accessing the public instance of idealImmunoTP

To use idealImmunoTP without installing any additional software, you can access the public instance of the Shiny Server made available at the Institute of Medical Biostatistics, Epidemiology and Informatics (IMBEI) in Mainz.

This resource is accessible at this address:

http://shiny.imbei.uni-mainz.de:3838/ideal

Deploying to a Shiny Server

A deployment-oriented version of the package is available at https://github.com/federicomarini/ideal_serveredition. This repository contains also detailed instruction to setup the running instance of a Shiny Server, where ideal can be run without further installation for the end-users.

Contact

For additional details regarding the functions of ideal, please consult the documentation or write an email to [email protected].

Bug reports/Issues/New features

Please use https://github.com/federicomarini/ideal/issues for reporting bugs, issues or for suggesting new features to be implemented.