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pydnaweb

A flask app that make selected pydna functionality available as a web service.

  • The WebPCR simulator.
  • Primer Tm calculator based on The Bio.SeqUtils.MeltingTemp module.
  • Primer designer. Design two flanking primers, given a template sequence.
  • Assembly primer designer. Design tailed primers for assembly by HR.
  • Assembly simulator. Assemble a series of sequences by shared homology.

webpcr

blogpost

Installed on pythonanywhere like this:

mkvirtualenv --python=/usr/bin/python3.9 my-virtualenv
pip install --upgrade flask flask-wtf wtforms pydna
git clone https://github.com/BjornFJohansson/pydnaweb.git

Settings uder the "Web" tab in python anywhere:

settings

Settings in the /var/www/pydna_pythonanywhere_com_wsgi.py file:

wsgi

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Online PCR simulator, Tm calculator and primer designer and more!

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