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This is a great tool however I'm running into a hurdle on the final step - regarding the multiQC reporting step. It appears to not be finding the multiqc config file?
jovyan@rlmt278-scdownstream-0:~/osteomics/projects/scdownstream$ nextflow run /home/jovyan/osteomics/projects/scdownstream/scdownstream/ --input /home/jovyan/osteomics/projects/scdownstream/scdownstream/sample_sheet.csv --outdir ./results -c /cache-juicefs/nextflow-workspace/test/work/marcus_config.config --preprocess_only TRUE --ambient_removal soupx -resume
N E X T F L O W ~ version 24.04.4
Launching `/home/jovyan/osteomics/projects/scdownstream/scdownstream/main.nf` [happy_lichterman] DSL2 - revision: 456139e594
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`._,._,'
nf-core/scdownstream 0.0.1dev
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Input/output options
input : /home/jovyan/osteomics/projects/scdownstream/scdownstream/sample_sheet.csv
outdir : ./results
Pipeline options
preprocess_only: true
ambient_removal: soupx
Core Nextflow options
runName : happy_lichterman
containerEngine: docker
launchDir : /home/jovyan/osteomics/projects/scdownstream
workDir : /cache-juicefs/nextflow-workspace/work
projectDir : /home/jovyan/osteomics/projects/scdownstream/scdownstream
userName : jovyan
profile : standard
configFiles :
!! Only displaying parameters that differ from the pipeline defaults !!
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* The nf-core framework
https://doi.org/10.1038/s41587-020-0439-x
* Software dependencies
https://github.com/nf-core/scdownstream/blob/master/CITATIONS.md
WARN: The following invalid input values have been detected:
* --max_memory: 1200.GB
* --max_cpus: 14
* --max_time: 7.d
executor > k8s (4)
[- ] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:SCANPY_READH5 -
[94/e0d742] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:ADATA_READRDS (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[- ] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:ADATA_READCSV -
[a5/e012e5] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:ADATA_UNIFY (SAMN14430801) [100%] 4 of 4, cached: 4 ✔
[2a/142145] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:GET_UNFILTERED_SIZE (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[- ] NFC…SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:EMPTY_DROPLET_REMOVAL:CELLBENDER_REMOVEBACKGROUND -
[- ] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:EMPTY_DROPLET_REMOVAL:ADATA_BARCODES -
[d4/39273a] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:GET_FILTERED_SIZE (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[56/8db6a1] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:QC_RAW (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[32/6c1937] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:AMBIENT_RNA_REMOVAL:SOUPX (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[a8/a6515b] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:SCANPY_FILTER (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[0b/799ebf] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:GET_THRESHOLDED_SIZE (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[8b/3178ae] NFC…_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:DOUBLET_DETECTION:SCANPY_SCRUBLET (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[fb/954257] NFC…_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:DOUBLET_DETECTION:DOUBLET_REMOVAL (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[ca/ef2870] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:GET_DEDOUBLETED_SIZE (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[3c/3b9bac] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:QC_FILTERED (SAMN14430799) [100%] 2 of 2, cached: 2 ✔
[69/40a1d5] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:COLLECT_SIZES (sizes) [100%] 1 of 1, cached: 1 ✔
[04/152249] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:MULTIQC [ 75%] 3 of 4, failed: 3, retries: 3
[79/eb0e4e] NOTE: Process `NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:MULTIQC` terminated with an error exit status (2) -- Execution is retried (1)
[ea/158996] NOTE: Process `NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:MULTIQC` terminated with an error exit status (2) -- Execution is retried (2)
[77/661a8e] NOTE: Process `NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:MULTIQC` terminated with an error exit status (2) -- Execution is retried (3)
ERROR ~ Error executing process > 'NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:MULTIQC'
Caused by:
Process `NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:MULTIQC` terminated with an error exit status (2)
Command executed:
multiqc \
--force \
\
--config multiqc_config.yml \
\
executor > k8s (4)
[- ] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:SCANPY_READH5 -
[94/e0d742] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:ADATA_READRDS (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[- ] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:ADATA_READCSV -
[a5/e012e5] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:ADATA_UNIFY (SAMN14430801) [100%] 4 of 4, cached: 4 ✔
[2a/142145] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:GET_UNFILTERED_SIZE (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[- ] NFC…SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:EMPTY_DROPLET_REMOVAL:CELLBENDER_REMOVEBACKGROUND -
[- ] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:EMPTY_DROPLET_REMOVAL:ADATA_BARCODES -
[d4/39273a] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:GET_FILTERED_SIZE (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[56/8db6a1] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:QC_RAW (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[32/6c1937] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:AMBIENT_RNA_REMOVAL:SOUPX (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[a8/a6515b] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:SCANPY_FILTER (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[0b/799ebf] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:GET_THRESHOLDED_SIZE (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[8b/3178ae] NFC…_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:DOUBLET_DETECTION:SCANPY_SCRUBLET (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[fb/954257] NFC…_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:DOUBLET_DETECTION:DOUBLET_REMOVAL (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[ca/ef2870] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:GET_DEDOUBLETED_SIZE (SAMN14430801) [100%] 2 of 2, cached: 2 ✔
[3c/3b9bac] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:QC_FILTERED (SAMN14430799) [100%] 2 of 2, cached: 2 ✔
[69/40a1d5] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:PREPROCESS:COLLECT_SIZES (sizes) [100%] 1 of 1, cached: 1 ✔
[04/152249] NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:MULTIQC [100%] 4 of 4, failed: 4, retries: 3 ✘
[79/eb0e4e] NOTE: Process `NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:MULTIQC` terminated with an error exit status (2) -- Execution is retried (1)
[ea/158996] NOTE: Process `NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:MULTIQC` terminated with an error exit status (2) -- Execution is retried (2)
[77/661a8e] NOTE: Process `NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:MULTIQC` terminated with an error exit status (2) -- Execution is retried (3)
ERROR ~ Error executing process > 'NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:MULTIQC'
Caused by:
Process `NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:MULTIQC` terminated with an error exit status (2)
Command executed:
multiqc \
--force \
\
--config multiqc_config.yml \
\
\
\
\
\
.
cat <<-END_VERSIONS > versions.yml
"NFCORE_SCDOWNSTREAM:SCDOWNSTREAM:MULTIQC":
multiqc: $( multiqc --version | sed -e "s/multiqc, version //g" )
END_VERSIONS
Command exit status:
2
Command output:
(empty)
Command error:
/// MultiQC 🔍 v1.25.1
Usage: multiqc [OPTIONS] [ANALYSIS DIRECTORY]
This is MultiQC v1.25.1
For more help, run 'multiqc --help' or visit http://multiqc.info
╭─ Error ──────────────────────────────────────────────────────────────────────╮
│ Invalid value for '-c' / '--config': Path 'multiqc_config.yml' does not │
│ exist. │
╰──────────────────────────────────────────────────────────────────────────────╯
Work dir:
/cache-juicefs/nextflow-workspace/work/04/152249a7d6ba23010d2d6e46c9d33a
Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
-- Check '.nextflow.log' file for details
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting
-- Check '.nextflow.log' file for details
-[nf-core/scdownstream] Pipeline completed with errors-
Command used and terminal output
No response
Relevant files
No response
System information
No response
The text was updated successfully, but these errors were encountered:
Description of the bug
Hi,
This is a great tool however I'm running into a hurdle on the final step - regarding the multiQC reporting step. It appears to not be finding the multiqc config file?
Command used and terminal output
No response
Relevant files
No response
System information
No response
The text was updated successfully, but these errors were encountered: