diff --git a/modules/nf-core/sylph/profile/environment.yml b/modules/nf-core/sylph/profile/environment.yml
new file mode 100644
index 00000000000..fd9d6bf1294
--- /dev/null
+++ b/modules/nf-core/sylph/profile/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - "bioconda::sylph=0.7.0"
diff --git a/modules/nf-core/sylph/profile/main.nf b/modules/nf-core/sylph/profile/main.nf
new file mode 100644
index 00000000000..2bd20e60fc6
--- /dev/null
+++ b/modules/nf-core/sylph/profile/main.nf
@@ -0,0 +1,52 @@
+process SYLPH_PROFILE {
+    tag "$meta.id"
+    label 'process_high'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/sylph:0.7.0--h919a2d8_0' :
+        'biocontainers/sylph:0.7.0--h919a2d8_0' }"
+
+    input:
+    tuple val(meta), path(reads)
+    path(pre_sketched_files)
+
+    output:
+    tuple val(meta), path('*.tsv'), emit: profile_out
+    path "versions.yml"           , emit: versions
+
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
+    """
+    sylph profile \\
+          $args \\
+          $reads \\
+          $pre_sketched_files\\
+          -o ${prefix}.tsv
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        sylph: \$(sylph -V|awk '{print \$2}')
+    END_VERSIONS
+
+    """
+
+    stub:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.tsv
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        sylph: \$(sylph -V|awk '{print \$2}')
+    END_VERSIONS
+    """
+
+}
diff --git a/modules/nf-core/sylph/profile/meta.yml b/modules/nf-core/sylph/profile/meta.yml
new file mode 100644
index 00000000000..88eda04c0a3
--- /dev/null
+++ b/modules/nf-core/sylph/profile/meta.yml
@@ -0,0 +1,56 @@
+name: "sylph_profile"
+description: Sylph profile command for taxonoming profiling
+keywords:
+  - profile
+  - metagenomics
+  - sylph
+  - classification
+tools:
+  - sylph:
+      description: Sylph quickly enables querying of genomes against even low-coverage
+        shotgun metagenomes to find nearest neighbour ANI.
+      homepage: https://github.com/bluenote-1577/sylph
+      documentation: https://github.com/bluenote-1577/sylph
+      tool_dev_url: https://github.com/bluenote-1577/sylph
+      doi: 10.1038/s41587-024-02412-y
+      licence: ["MIT"]
+      identifier: biotools:sylph
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'test', single_end:false ]`
+    - reads:
+        type: file
+        description: |
+          List of input FastQ/FASTA files of size 1 and 2 for single-end and paired-end data,
+          respectively. They are automatically sketched to .sylsp/.syldb
+  - - pre_sketched_files:
+        type: file
+        description: Pre-sketched *.syldb/*.sylsp files
+        pattern: "*.{syldb,sylsp}"
+output:
+  - profile_out:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+          pattern: "*tsv"
+      - "*.tsv":
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+          pattern: "*tsv"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
+authors:
+  - "@jiahang1234"
+  - "@sofstam"
+maintainers:
+  - "@sofstam"
diff --git a/modules/nf-core/sylph/profile/tests/main.nf.test b/modules/nf-core/sylph/profile/tests/main.nf.test
new file mode 100644
index 00000000000..de0f0e35ba1
--- /dev/null
+++ b/modules/nf-core/sylph/profile/tests/main.nf.test
@@ -0,0 +1,86 @@
+nextflow_process {
+
+    script "../main.nf"
+    process "SYLPH_PROFILE"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "sylph"
+    tag "sylph/profile"
+
+    test("sarscov2 illumina single-end [fastq_gz]") {
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:true ], // meta map
+                             [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
+                           ]
+                input[1] = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.versions,
+                    process.out.profile_out.collect { file(it[1]).readLines().contains("complete genome") },
+                ).match()
+            }
+            )
+        }
+    }
+
+    test("sarscov2 illumina paired-end [fastq_gz]") {
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                             [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                               file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
+                           ]
+                input[1] = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.versions,
+                    process.out.profile_out.collect { file(it[1]).readLines().contains("complete genome") },
+                ).match()
+            }
+            )
+        }
+    }
+
+    test("sarscov2 illumina paired-end [fastq_gz]-stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                             [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                               file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
+                           ]
+                input[1] = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.versions,
+                    process.out.profile_out.collect { file(it[1]).readLines().contains("complete genome") },
+                ).match()
+            }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/sylph/profile/tests/main.nf.test.snap b/modules/nf-core/sylph/profile/tests/main.nf.test.snap
new file mode 100644
index 00000000000..da7a092b33a
--- /dev/null
+++ b/modules/nf-core/sylph/profile/tests/main.nf.test.snap
@@ -0,0 +1,59 @@
+{
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,7b5a545483277cc0ff9189f8891e737f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-12-05T10:06:07.353254"
+    },
+    "sarscov2 illumina paired-end [fastq_gz]": {
+        "content": [
+            [
+                "versions.yml:md5,7b5a545483277cc0ff9189f8891e737f"
+            ],
+            [
+                false
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-12-05T10:31:09.774052"
+    },
+    "sarscov2 illumina single-end [fastq_gz]": {
+        "content": [
+            [
+                "versions.yml:md5,7b5a545483277cc0ff9189f8891e737f"
+            ],
+            [
+                false
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-12-05T10:29:46.733197"
+    },
+    "sarscov2 illumina paired-end [fastq_gz]-stub": {
+        "content": [
+            [
+                "versions.yml:md5,7b5a545483277cc0ff9189f8891e737f"
+            ],
+            [
+                false
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-12-05T10:31:16.953204"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/sylph/sketch/meta.yml b/modules/nf-core/sylph/sketch/meta.yml
index 7a9abdf85b1..9d3645c959a 100644
--- a/modules/nf-core/sylph/sketch/meta.yml
+++ b/modules/nf-core/sylph/sketch/meta.yml
@@ -35,7 +35,6 @@ output:
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-          pattern: "my_sketches/*.sylsp"
       - my_sketches/*.sylsp:
           type: map
           description: |