Determination of Soil Microbial Biodiversity

[rima-bio]

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[rima-bio]

1. Through amplicon sequencing (Illumina MiSeq):
   The diversity of soil bacteria and fungi was analyzed through amplicon sequencing (Illumina MiSeq). A total of 10 g of frozen soil samples were cooled using liquid nitrogen and ground using a mortar and pestle. Soil DNA (n = 435) was extracted using a DNA Isolation Kit (Powersoil, MoBio Laboratories, Carlsbad, CA, USA). A portion of the bacterial 16S and eukaryotic 18S rRNA genes were sequenced using the 515F/806R and Euk1391f/EukBr primer sets, respectively (https://doi.org/10.1038/s41396-021-00906-0.