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Read image files

img_path_ls = glob.glob(img_dir + '/*.jpg') # or '/*.png'
for img_path in img_path_ls:
    img = cv2.imread(img_path)

Read frames from a video file

cap = cv2.VideoCapture(<video_file_path>)
ret, frame_rgb = cap.read()
while ret:
    cv2.imshow('frame_rgb', frame_rgb)
    cv2.waitKey(0)
    ret, frame_rgb = cap.read()