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flowjo_to_gatingset ignore range as defined in FCS files #155

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Close-your-eyes opened this issue Apr 4, 2024 · 3 comments
Open

flowjo_to_gatingset ignore range as defined in FCS files #155

Close-your-eyes opened this issue Apr 4, 2024 · 3 comments

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@Close-your-eyes
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Hello.

Is there a way to tell flowjo_to_gatingset that is should ignore the range keyword from FCS files? Similar to the truncate_max_range argument in flowCore::read.FCS?

I found a workaround by modifying the respective keywords in the FCS files. However, this seems to be generic problem / convenient feature.

Thanks.

@mikejiang
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are you referring to $PnR keywords? They are required according to the standard, Why would you want to ignore them?

@Close-your-eyes
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Yes I do mean these keywords. In my case it appears that the machine actually has resolution for higher values in fsc and ssc channels.

@mikejiang
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These keywords are used extensively in cytoverse tools, thus it is important to make sure they are accurate and consistent, which was what you already did

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