Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

boolean gate error #153

Open
kaitlinchaung opened this issue Nov 8, 2023 · 0 comments
Open

boolean gate error #153

kaitlinchaung opened this issue Nov 8, 2023 · 0 comments

Comments

@kaitlinchaung
Copy link

kaitlinchaung commented Nov 8, 2023

Hi, I am currently trying to use the gs-to-wsp function, but I am getting errors related to boolean gating. I can't find a relevant Issue or documentation pertaining this error, so I am unsure how to proceed.

My gating template does contain boolean gates.

Command:

sudo docker run \
    -v <path>/gs:/gs \
    -v <path>/out:/out \
    public.ecr.aws/x4k5d9i7/cytoverse/gs-to-wsp \
    --src=gs/ \
    --dest=/out/test_bbt.wsp

Error:

terminate called after throwing an instance of 'std::runtime_error'
  what():  isNot flag must be TRUE in 'Not' boolean gate!

The beginning of the gating template, where the CD3Neg gate is causing the failure:

alias,pop,parent,dims,gating_method,gating_args,collapseDataForGating,groupBy,preprocessing_method,preprocessing_args
CD14gate,+,root,CD14,gate_mindensity2,"gate_range=c(1,3)",,,,
CD56gate,+,root,CD56,gate_mindensity2,"gate_range=c(1,3)",,,,
CD3gate,+,root,CD3,gate_mindensity2,"gate_range=c(1,3)",,,,
CD19gate,+,root,CD19,gate_mindensity2,"gate_range=c(1,3)",,,,
CD3Neg,+,root,CD3,boolGate,!CD3gate,,,,
CD3Pos,+,root,CD3,boolGate,CD3gate,,,,

Thank you!

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant